dUTP / 250 μL

2′-Deoxyuridine 5′-triphosphate sodium salt solution is a 100 mM nucleotide triphosphate dissolved in H2O, widely employed in molecular biology applications. It acts as a crucial constituent for DNA synthesis during PCR and various enzymatic processes.

Application: Utilized as a substrate for DNA polymerases, 2′-deoxyuridine 5′-triphosphate sodium salt solution, with a concentration of 100 mM in H2O, is pivotal in supporting DNA amplification techniques such as PCR. Its incorporation ensures precise and efficient DNA replication in diverse molecular biology experiments.

Articles:

-An isotopic assay of dUTPase activity based on coupling with thymidylate synthase.

First Published: September 30th, 1999

B. Gołos and W. Rode

https://doi.org/10.18388/abp.1999_4155

-Influence of residual uracil-DNA glycosylase activity on the electrophoretic migration of dUTP-containing PCR products

First Published: February 1, 1999

Michael Ritzler, Inge Perschil, Martin Altwegg

https://doi.org/10.1016/S0167-7012(98)00104-3 

- Mechanism of Cell Death following Thymidylate Synthase Inhibition: 2′-Deoxyuridine-5′-triphosphate Accumulation, DNA Damage, and Growth Inhibition following Exposure to CB3717 and Dipyridamole

First Published: February 13, 1991

Nicola J. Curtin, Adrian L. Harris, G. Wynne Aherne.

-dUTP incorporation into genomic DNA is linked to transcription in yeast

Published: May 17, 2009

https://doi.org/10.1038/nature08033