AMP-PNP / 25 MG

AMP-PNP, an analog of ATP, features a unique molecular structure: 5'-Adenylic acid, monoanhydride with imidodiphosphoric acid. Distinguished by a nitrogen atom in place of the bridging oxygen between the beta and gamma phosphate, AMP-PNP serves as a potent competitive inhibitor. Its inhibitory effects extend to both soluble and membrane-bound mitochondrial ATPase, making it a valuable tool for studying ATP-dependent reactions in oxidative phosphorylation within various biological contexts. Renowned for stability and non-hydrolyzable properties, AMP-PNP stands as an essential component in molecular biology research, particularly in deciphering intricate mechanisms involving ATP interactions.

Application: AMP-PNP, recognized for its unique structure, acts as a robust competitive inhibitor, influencing both soluble and membrane-bound mitochondrial ATPase. Widely employed in molecular biology studies, it serves as a reliable surrogate for ATP, enabling precise investigations into ATP-dependent reactions. AMP-PNP's inhibitory prowess extends to crucial processes like oxidative phosphorylation, offering researchers a powerful tool to unravel the complexities of biological mechanisms.

Articles:

-A potential modification of the production of the essential amino acid: L-threonine

Cecile Petit, Younghwa Kim, Sungkwon Lee, Chong-Min Kang, Donald Ronning

First published: 03 October 2018 | https://doi.org/10.1096/fasebj.31.1_supplement.765.8

-Structure of AMP-PNP-bound vitamin B12 transporter BtuCD–F

Vladimir M. Korkhov, Samantha A. Mireku & Kaspar P. Locher
Published: 23 September 2012 | https://doi.org/10.1038/nature11442

-Structure of the multidrug ABC transporter Sav1866 from Staphylococcus aureus in complex with AMP-PNP

Roger J.P. Dawson, Kaspar P. Locher 

First published: 07 February 2007  |  https://doi.org/10.1016/j.febslet.2007.01.073